Tissue Culture

Tissue Culture Methods

I. TYPES OF CELLS GROWN IN CULTURE

Tissue last is a good deal a generic term that refers to both harmonium culture and cell culture and the terms atomic number 18 often used interchangeably. Cell cultures ar derived from either primary create from raw material explants or cell suspensions. Primary cell cultures typically depart have a finite life span in culture whereas continuous cell lines atomic number 18, by definition, abnormal and are often transformed cell lines.

II. WORK AREA AND EQUIPMENT

A. stratified point hoods. There are two types of laminar flow hoods, vertical and horizontal. The vertical hood, also known as a biology safety cabinet, is outdo for working with hazardous organisms since the aerosols that are generated in the hood are filtered out before they are released into the surrounding environment. Horizontal hoods are designed such that the send out flows directly at the performer hence they are non useful for working with hazardous organisms but are the best protection for your cultures. Both types of hoods have continuous displacement of blood line that passes through a HEPA (high efficiency particle) filter that removes particulates from the air.



In a vertical hood, the filtered air blows down from the top of the cabinet; in a horizontal hood, the filtered air blows out at the operator in a horizontal fashion. NOTE: these are non fume hoods and should not be used for volatile or explosive chemicals. They should also never be used for bacterial or fungal work. The hoods are equipped with a short-wave UV light that can be turned on for a few proceeding to sterilize the sur cases of the hood, but be aware(p) that only exposed surfaces will be accessible to the UV light. Do not put your hands or face near the hood when the UV light is on as the short wave light can cause undress and eye damage. The hoods should be turned on about 10-20 minutes before being used. Wipe down all surfaces with ethyl alcohol before and after each use. Keep the hood as free of clutter as possible because this will...If you want to fix a full essay, order it on our website: Orderessay



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