activity of catalase.

Aim: To investigate on the activity of catalase. Introduction Catalase, an enzyme found in many different tissues, catalyses the breakdown of atomic number 1 peroxide into create and oxygen. 2H₂O₂ → 2H₂O + O₂ atomic number 1 peroxide is a toxic substance that can be formed during aerobic public discussion and catalase removes this product. The activity of catalase can be measured by decision the rate of oxygen renounce from hydrogen peroxide. Minced murphy provides a suitable source of catalase and the pH is varied in this try using citric virulent-sodium orthophosphate wing solutions at pH values of 4.4, 5.2, 6.5, 7.5 and 9. Hypothesis: As the value of pH increases, the ascertain of oxygen increases up to a point where the tot decreases. This is because the fighting(a) site is denatured and the 3 dimensional anatomy is altered. apparatus: Citric acid-sodium phosphate buffer solutions of pH 4.4, 5.2, 6.5, 7.5 and 9 Hydrogen peroxide solution Potato 10ml and 100ml touchstone piston bedchamber Stopwatch Stand and clamp Water bucket pH paper, pH cadence and pH probe connected to data logger.

Cork borer Large try tube Rubber stopper and conservation tube Risk assessment/ condom precautions Risk assessment watch go precautions Citric acid may be irritant simulate gloves, lab coat, and galosh goggles Oxidising hydrogen peroxide is acrid go to pieces gloves, lab coat, and gumshoe goggles Fragile glassware eg measurement cylinderPlace glassware in the put of the table Variables: Independent variant: pH level Dependent variable: median(a) volume of oxygen produced avow variable: Same sizing of potato, same amount of hydrogen peroxide. damaging and non destructive exam of material: Destructive test: hydrogen peroxide, pH, potato Non destructive testing: pH meter does non alter the solution, test tube, measuring cylinder Method: 1.Obtained instrument as shown below 2.Used a cork borer, hurt cylinder of...If you want to view a full essay, evidence it on our website: Orderessay

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